Advancing male age differentially alters levels and localization patterns of PLCzeta in sperm and testes from different mouse strains

Sperm-specific phospholipase C zeta (PLCζ) initiates intracellular calcium (Ca2+) transients which drive a series of concurrent events collectively termed oocyte activation. Numerous investigations have linked abrogation and absence/reduction PLCζ with forms male infertility in humans where activation fails. However, very few studies examined potential relationships between advancing age, both are increasingly considered to be major effectors fertility. Initial efforts may hindered by inherent variability within the human population, alongside lack sufficient controllable repeats. Herein, utilizing immunoblotting, immunofluorescence, quantitative reverse transcription PCR (qRT-PCR) we for first time protein levels localization patterns sperm, mRNA testes, from mice at 8 weeks, 12 24 36 weeks two separate strains mice, C57BL/6 (B6; inbred) CD1 (outbred). Collectively, age generally diminished sperm respectively, when were examined. Furthermore, altered predominant pattern mouse younger exhibiting predominantly post-acrosomal, older post-acrosomal acrosomal populations PLCζ. specific such decline was strain-specific. our results demonstrate negative relationship patterns, indicating that aging different serve as useful models investigate cases subfertility humans.